کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
6482257 1415983 2018 45 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Proteomic profiling of Nostoc flagelliforme reveals the common mechanism in promoting polysaccharide production by different light qualities
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
سفارش ترجمه تخصصی
با تضمین قیمت و کیفیت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Proteomic profiling of Nostoc flagelliforme reveals the common mechanism in promoting polysaccharide production by different light qualities
چکیده انگلیسی
Different light qualities have been found to effectively promote polysaccharide production of cyanobacteria, to elucidate the underlying mechanism, the effects of four typical light qualities including ultraviolet radiation B (UVB), red light (R), blue light (B), and mixed light (R:B = 7:15) (RB) on polysaccharide production in Nostoc flagelliforme were comprehensively investigated with white fluorescent light as control. In this study, an iTRAQ-based proteomic approach was employed to explore the proteomes of N. flagelliforme, and 126, 177, 208, and 200 differentially expressed proteins were identified in UVB-, R-, B- and RB-treated N. flagelliforme. Functional classification analysis showed that differentially expressed proteins were mainly involved in photosynthesis, antioxidant activity, and carbohydrate metabolism. Analysis of differentially expressed proteins involved in polysaccharide synthesis revealed that different light qualities shared a common mechanism to promote polysaccharide production by altering carbon allocation and increasing carbon flow into sugar nucleotide synthesis pathway. Activity assay of selected enzymes further verified the proteomic analysis. Our results provide novel insights into the molecular mechanisms of light quality affecting polysaccharide production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 132, 15 April 2018, Pages 68-78
نویسندگان
, , , , , , , ,
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
سفارش ترجمه تخصصی
با تضمین قیمت و کیفیت