کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6482257 | 1415983 | 2018 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic profiling of Nostoc flagelliforme reveals the common mechanism in promoting polysaccharide production by different light qualities
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کلمات کلیدی
PMMPGMR5PUDP-GlcAPFKUDP-xyloseGlycerateF6P3PGRuBPADP-riboseNostoc flagelliformeUGPaseUDP-GalG1PUDP-XylRU5PX5P6PGxylulose-5-phosphateG6PM6PGDP-FucFBPUDP-galactose - UDP-گالاکتوزUDP-glucose pyrophosphorylase - UDP-گلوکز pyrophosphorylaseUDP-glucuronic acid - اسید UDP-گلوکورونیکGDP-mannose - تولید انبوه انارGDP-Man - تولید ناخالص داخلی مردribose-5-phosphate - ریبوز 5-فسفاتribulose-5-phosphate - ریوبولوز 5-فسفاتCyanobacteria - سیانوباکتریهاPMI - شرکتهای کوچک و متوسطGAP - شکافfructose-6-phosphate - فروکتوز 6-فسفاتPhosphomannomutase - فسفامانوموتازPhosphofructokinase - فسفوفروتکتینازPhosphomannose isomerase - فسفومونوز ایزومرازphosphoglucomutase - فسفوگلوکوموتازMannose-1-phosphate - مانوز 1-فسفاتmannose-6-phosphate - مانوز 6-فسفاتProteomics - پروتئومیکسCapsular polysaccharide - پلی ساکارید کپسولGDP-d-mannose pyrophosphorylase - پیرو فسفریلاس تولید ناخالص داخلی منیزیمPolysaccharide - چندقندی ها یا پلیساکاریدهاLight quality - کیفیت نورGlu - گلوGlucose - گلوکزglucose-1-phosphate - گلوکز 1-فسفاتglucose-6-phosphate - گلوکز 6-فسفاتUDP-glucose - گلوکز UDPglyceraldehyde-3-phosphate - گلیسرالیدید-3-فسفات
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Proteomic profiling of Nostoc flagelliforme reveals the common mechanism in promoting polysaccharide production by different light qualities Proteomic profiling of Nostoc flagelliforme reveals the common mechanism in promoting polysaccharide production by different light qualities](/preview/png/6482257.png)
چکیده انگلیسی
Different light qualities have been found to effectively promote polysaccharide production of cyanobacteria, to elucidate the underlying mechanism, the effects of four typical light qualities including ultraviolet radiation B (UVB), red light (R), blue light (B), and mixed light (R:Bâ¯=â¯7:15) (RB) on polysaccharide production in Nostoc flagelliforme were comprehensively investigated with white fluorescent light as control. In this study, an iTRAQ-based proteomic approach was employed to explore the proteomes of N. flagelliforme, and 126, 177, 208, and 200 differentially expressed proteins were identified in UVB-, R-, B- and RB-treated N. flagelliforme. Functional classification analysis showed that differentially expressed proteins were mainly involved in photosynthesis, antioxidant activity, and carbohydrate metabolism. Analysis of differentially expressed proteins involved in polysaccharide synthesis revealed that different light qualities shared a common mechanism to promote polysaccharide production by altering carbon allocation and increasing carbon flow into sugar nucleotide synthesis pathway. Activity assay of selected enzymes further verified the proteomic analysis. Our results provide novel insights into the molecular mechanisms of light quality affecting polysaccharide production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 132, 15 April 2018, Pages 68-78
Journal: Biochemical Engineering Journal - Volume 132, 15 April 2018, Pages 68-78
نویسندگان
Pei-pei Han, Rong-jun Guo, Shi-gang Shen, Rong-rong Yan, Yi-kai Wu, Shun-yu Yao, Hui-yan Wang, Shi-ru Jia,