کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6482546 | 1415895 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel method for preparing high purity Actinobacillus succinogenes stock and its long-term acid production in a packed bed reactor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A. succinogenes is susceptible to contamination by other lactate producing bacteria. This reduces stability and makes long-term A. succinogenes fermentation difficult. In this study, the contaminated A. succinogenes culture, where the contaminant was shown to be ubiquitous Staphylococcus epidermidis, can be easily rescued by using xylose as the sole carbon source. The stability of purified A. succinogenes was verified in continuous fermentation using packed bed reactor while the performance of acid productions was investigated. While MgCO3 was used as a pH buffer and CO2 source during the continuous fermentation, the problem of MgCO3 clogging in a packed bed reactor was solved by oleyl alcohol reflux. This raised operation time from 344 to 1788â¯h. The provision of 30â¯g/L glucose and 40â¯g/L xylose as carbon sources resulted in a main metabolite yield of 91.7â¯Â±â¯3.2% lactate and 55.3â¯Â±â¯3.0% succinate with a stable productivity of 16.9â¯Â±â¯1.7 and 1.5â¯Â±â¯0.1â¯g·Lâ1·hâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology Reports - Volume 2, June 2018, Pages 62-68
Journal: Bioresource Technology Reports - Volume 2, June 2018, Pages 62-68
نویسندگان
Yu-Sheng Chiang, Yu-Yuan Kuo, Si-Yu Li,