کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6483993 132 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expansion of human mesenchymal stem cells in fibrous bed bioreactor
ترجمه فارسی عنوان
گسترش سلول های بنیادی مزانشیمی انسانی در بیوراکتور تخت فیبری
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Expansion of human mesenchymal stem cells (hMSCs) in bioreactor while preserving their innate properties is important in translation of hMSC-based therapy to clinical applications. The present study investigates the feasibility of hMSC expansion in a 2.5 L CelliGen® 310 Bioreactor packed with Fibra-Cel® disks. After 9 days of expansion, a 9.2-fold increase in cell number with the population doubling (PD) time of 2.8 days (67.2 h) was achieved and that the specific glucose consumption and lactate production were measured to be 12.48 pmol/cell/day and 20.95 pmol/cell/day, respectively. hMSCs harvested from the bioreactor maintained their properties based on the analysis of phenotypic surface markers, colony forming unit-fibroblasts (CFU-F) number, and multilineage differentiation ability. The results demonstrate the feasibility and the potential of the fibrous bed bioreactor for large scale hMSC expansion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 108, 15 April 2016, Pages 51-57
نویسندگان
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