کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6488150 1416359 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An OPAA enzyme mutant with increased catalytic efficiency on the nerve agents sarin, soman, and GP
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
An OPAA enzyme mutant with increased catalytic efficiency on the nerve agents sarin, soman, and GP
چکیده انگلیسی
The wild-type OPAA enzyme has relatively high levels of catalytic activity against several organophosphate G-type nerve agents. A series of mutants containing replacement amino acids at the OPAA Y212, V342, and I215 sites showed several fold enhanced catalytic efficiency on sarin, soman, and GP. One mutant, Y212F/V342L, showed enhanced stereospecificity on sarin and that enzyme along with a phosphotriesterase mutant, GWT, which had the opposite stereospecificity, were used to generate enriched preparations of each sarin enantiomer. Inhibition of acetylcholinesterase by the respective enantioenriched sarin solutions subsequently provided identification of the sarin enantiomers as separated by normal phase enantioselective liquid chromatography coupled with atmospheric pressure chemical ionization-mass spectrometry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 112, May 2018, Pages 65-71
نویسندگان
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