Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

- Lipase immobilization rate on octyl supports is reduced at high ionic strength.
- Octyl-lipase is more rapidly inhibited by D-pNPP than covalent preparations.
- Octyl-lipase inhibition by D-pNPP did not depend on ionic strength.
- Octyl-lipase activity is less depended on ionic strength.
- Lipase activity is not only depended on catalytic Ser exposition.