Kinetics, aggregation behavior and optimization of the fractionation of whey protein isolate with hydrochloric acid

► Fractionation of concentrated whey protein isolate with hydrochloric acid. ► Analysis of α-LA aggregation behaviors and precipitation kinetics. ► Quantitative effects of processing time, pH, temperature and concentration. ► Selective α-LA precipitation obtained on narrow pH and T range. ► Process optimization for highest quality and yield of enriched protein fractions.