کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490159 | 1416963 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of a new microbial Pictet-Spenglerase NscbB affording the β-carboline skeletons from Nocardiopsis synnemataformans DSM 44143
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplant patient derived) by homologous alignment with its well-characterized counterpart McbB. The biochemical analysis showed that NscbB could catalyze l-tryptophan (KMâ¯=â¯89.64â¯Â±â¯8.69â¯Î¼M) and methylglyoxal (KMâ¯=â¯147.70â¯Â±â¯16.38â¯Î¼M), without cofactors, to form the two βC skeletons 1-acetyl-3-carboxy-β-carboline and 1-acetyl-β-carboline in vitro. Additionally, the heterologous expression of nscbB in E. coli BL21 (DE3) revealed the efficient bioproductivity of NscbB to bioproduce two βC skeletons, 1-acetyl-3-carboxy-β-carboline (5.5â¯mg/L) and 1-acetyl-β-carboline (3.1â¯mg/L), within 16â¯h fermentation. These results demonstrate NscbB is a novel and practical microbial PSase, which is useful for future bioactive βC alkaloid exploitation and development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 281, 10 September 2018, Pages 137-143
Journal: Journal of Biotechnology - Volume 281, 10 September 2018, Pages 137-143
نویسندگان
Qi Chen, Shaofei Zhang, Yunchang Xie,