کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6490510 43365 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Screening and characterization of molecules that modulate the biological activity of IFNs-I
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Screening and characterization of molecules that modulate the biological activity of IFNs-I
چکیده انگلیسی
Type I Interferons (IFNs-I) are species-specific glycoproteins which play an important role as primary defence against viral infections and that can also modulate the adaptive immune system. In some autoimmune diseases, interferons (IFNs) are over-produced. IFNs are widely used as biopharmaceuticals for a variety of cancer indications, chronic viral diseases, and for their immuno-modulatory action in patients with multiple sclerosis; therefore, increasing their therapeutic efficiency and decreasing their side effects is of high clinical value. In this sense, it is interesting to find molecules that can modulate the activity of IFNs. In order to achieve that, it was necessary to establish a simple, fast and robust assay to analyze numerous compounds simultaneously. We developed four reporter gene assays (RGAs) to identify IFN activity modulator compounds by using WISH‐Mx2/EGFP, HeLa-Mx2/EGFP, A549-Mx2/EGFP, and HEp2‐Mx2/EGFP reporter cell lines (RCLs). All of them present a Z′ factor higher than 0.7. By using these RGAs, natural and synthetic compounds were analyzed simultaneously. A total of 442 compounds were studied by the Low Throughput Screening (LTS) assay using the four RCLs to discriminate between their inhibitory or enhancing effects on IFN activity. Some of them were characterized and 15 leads were identified. Finally, one promising candidate with enhancing effect on IFN-α/-β activity and five compounds with inhibitory effect were described.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 233, 10 September 2016, Pages 6-16
نویسندگان
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