Characterization of recombinantly expressed dihydroxy-acid dehydratase from Sulfobus solfataricus-A key enzyme for the conversion of carbohydrates into chemicals

The total turnover numbers for all of the three reactions was found to be 35.5 × 103 ± 1.0 × 103 for the conversion of d-gluconate to 2-keto-3-deoxygluconate (KDG), 28.2 × 103 ± 0.8 × 103 for DHIV to 2-ketovalerate (KIV) and 943 ± 0.28 × 102 for d-glycerate to pyruvate. With activated SsDHAD these values could be further increased 5- and 4-fold for the d-gluconate and d-glycerate conversion, respectively.