کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490994 | 43389 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genetic profiling of yeast industrial strains using in situ comparative genomic hybridization (CGH)
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Genetic profiling of yeast industrial strains using in situ comparative genomic hybridization (CGH) Genetic profiling of yeast industrial strains using in situ comparative genomic hybridization (CGH)](/preview/png/6490994.png)
چکیده انگلیسی
The genetic differences and changes in genomic stability may affect fermentation processes involving baker's, brewer's and wine yeast strains. Thus, it seems worthwhile to monitor the changes in genomic DNA copy number of industrial strains. In the present study, we developed an in situ comparative genomic hybridization (CGH) to investigate the ploidy and genetic differences between selected industrial yeast strains. The CGH-based system was validated using the laboratory Saccharomyces cerevisiae yeast strains (haploid BY4741 and diploid BY4743). DNA isolated from BY4743 cells was considered a reference DNA. The ploidy and DNA gains and losses of baker's, brewer's and wine strains were revealed. Taken together, the in situ CGH was shown a helpful molecular tool to identify genomic differences between yeast industrial strains. Moreover, the in situ CGH-based system may be used at the single-cell level of analysis to supplement array-based techniques and high-throughput analyses at the population scale.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 210, 20 September 2015, Pages 52-56
Journal: Journal of Biotechnology - Volume 210, 20 September 2015, Pages 52-56
نویسندگان
Maciej Wnuk, Anita Panek, Ewelina Golec, Michal Magda, Anna Deregowska, Jagoda Adamczyk, Anna Lewinska,