کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491007 | 43389 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparing effects of perfusion and hydrostatic pressure on gene profiles of human chondrocyte.
ترجمه فارسی عنوان
مقایسه اثرات پرفیوژن و فشار هیدرواستاتیک بر پروفایل ژن کانتورسیت انسانی.
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
پرفیوژن، فشار هیدرواستاتیک، بیوراکتور، ترتیب دهی، کاندروسیت های انسانی،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Hydrostatic pressure and perfusion have been shown to regulate the chondrogenic potential of articular chondrocytes. In order to compare the effects of hydrostatic pressure plus perfusion (HPP) and perfusion (P) we investigated the complete gene expression profiles of human chondrocytes under HPP and P. A simplified bioreactor was constructed to apply loading (0.1Â MPa for 2Â h) and perfusion (2Â ml) through the same piping by pressurizing the medium directly. High-density monolayer cultures of human chondrocytes were exposed to HPP or P for 4 days. Controls (C) were maintained in static cultures. Gene expression was evaluated by sequencing (RNAseq) and quantitative real-time PCR analysis. Both treatments changed gene expression levels of human chondrocytes significantly. Specifically, HPP and P increased COL2A1 expression and decreased COL1A1 and MMP-13 expression. Despite of these similarities, RNAseq revealed a list of cartilage genes including ACAN, ITGA10 and TNC, which were differentially expressed by HPP and P. Of these candidates, adhesion related molecules were found to be upregulated in HPP. Both HPP and P treatment had beneficial effects on chondrocyte differentiation and decreased catabolic enzyme expression. The study provides new insight into how hydrostatic pressure and perfusion enhance cartilage differentiation and inhibit catabolic effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 210, 20 September 2015, Pages 59-65
Journal: Journal of Biotechnology - Volume 210, 20 September 2015, Pages 59-65
نویسندگان
Ge Zhu, Susanne Mayer-Wagner, Christian Schröder, Matthias Woiczinski, Helmut Blum, Ilaria Lavagi, Stefan Krebs, Julia I. Redeker, Andreas Hölzer, Volkmar Jansson, Oliver Betz, Peter E. Müller,