کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491029 | 43389 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Small RNAs targeting the 5â² end of the viral polymerase gene segments specifically interfere with influenza type A virus replication
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Small RNAs targeting the 5â² end of the viral polymerase gene segments specifically interfere with influenza type A virus replication Small RNAs targeting the 5â² end of the viral polymerase gene segments specifically interfere with influenza type A virus replication](/preview/png/6491029.png)
چکیده انگلیسی
Human and avian influenza A viruses, associated with seasonal epidemics and occasionally with pandemics, have a high impact on public health. The development of new antivirals to counteract the emergence of drug resistant influenza virus variants is a main concern. The aim of this study was to develop systems for the efficient and stable expression of small therapeutic RNAs into influenza virus infected cells in order to get further insights on the efficacy of nucleic acid-based antiviral strategies. To this end, lentiviral vectors expressing either microRNAs or antisense-RNAs targeting the 5â² end of the PA, PB1 and PB2 influenza virus genomic sequences were generated. Derivative recombinant lentiviral particles were employed to transduce the influenza virus highly susceptible human alveolar basal epithelial A549 cells. The expression of both RNA molecules led to a reduction up to 3 logs of the viral titer when transduced A549 cells were challenged with different human and avian subtypes of influenza type A virus. Importantly, no inhibition of influenza type B virus was observed. Overall our data support the development of nucleic acid-based antiviral strategies to control human and avian influenza A virus infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 210, 20 September 2015, Pages 85-90
Journal: Journal of Biotechnology - Volume 210, 20 September 2015, Pages 85-90
نویسندگان
Francesco Martelli, Cristiano Salata, Arianna Calistri, Cristina Parolin, Alberta Azzi, Giorgio Palù, Simone Giannecchini,