کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491076 | 43399 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of optically pure l-phenyllactic acid by using engineered Escherichia coli coexpressing l-lactate dehydrogenase and formate dehydrogenase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Production of optically pure l-phenyllactic acid by using engineered Escherichia coli coexpressing l-lactate dehydrogenase and formate dehydrogenase Production of optically pure l-phenyllactic acid by using engineered Escherichia coli coexpressing l-lactate dehydrogenase and formate dehydrogenase](/preview/png/6491076.png)
چکیده انگلیسی
l-Phenyllactic acid (l-PLA) is a novel antiseptic agent with broad and effective antimicrobial activity. In addition, l-PLA has been used for synthesis of poly(phenyllactic acid)s, which exhibits better mechanical properties than poly(lactic acid)s. However, the concentration and optical purity of l-PLA produced by native microbes was rather low. An NAD-dependent l-lactate dehydrogenase (l-nLDH) from Bacillus coagulans NL01 was confirmed to have a good ability to produce l-PLA from phenylpyruvic acid (PPA). In the present study, l-nLDH gene and formate dehydrogenase gene were heterologously coexpressed in Escherichia coli. Through two coupled reactions, 79.6Â mM l-PLA was produced from 82.8Â mM PPA in 40Â min and the enantiomeric excess value of l-PLA was high (>99%). Therefore, this process suggested a promising alternative for the production of chiral l-PLA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 207, 10 August 2015, Pages 47-51
Journal: Journal of Biotechnology - Volume 207, 10 August 2015, Pages 47-51
نویسندگان
Zhaojuan Zheng, Mingyue Zhao, Ying Zang, Ying Zhou, Jia Ouyang,