In vitro co-culture model of medulloblastoma and human neural stem cells for drug delivery assessment

- Physiologically relevant in vitro medulloblastoma model is described.
- Human-based normal and tumour tissue were cultured together in three dimensions.
- A universally applicable cell labelling and analysis technique was developed.
- Flow cytometry and multiphoton microscopy were used to quantify each population.
- Etoposide was used to model local brain-targeted chemotherapy.