کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491165 | 43391 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunosensor for trace penicillin G detection in milk based on supported bilayer lipid membrane modified with gold nanoparticles
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
In this work, we developed an immunosensor for electrochemical detection of penicillin G at trace level. The biosensor was fabricated by immobilizing anti-penicillin G in a supported bilayer lipid membrane (s-BLM) modified with gold nanoparticles, and the modified electrodes were characterized by the scanning electron microscope (SEM), cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was able to detect penicillin G with a linear correlation ranging from 3.34Â ÃÂ 10â3Â ng/L to 3.34Â ÃÂ 103Â ng/L and a detection limit of 2.7Â ÃÂ 10â4Â ng/L, much lower than the maximum residue limit (MRL) of penicillin G in milk (4Â ppb, equal to 4Â ÃÂ 103Â ng/L) set out by the European Union. The mean coefficient variation (CV) of the intra-assays and the inter-assays were 5.4% and 7.7%, respectively. In addition, the concentration of penicillin G in milk samples determined by this biosensor was in good agreement with that determined by high performance liquid chromatography (HPLC) assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 203, 10 June 2015, Pages 97-103
Journal: Journal of Biotechnology - Volume 203, 10 June 2015, Pages 97-103
نویسندگان
Han Li, Bing Xu, Danqi Wang, Yin Zhou, Hongling Zhang, Wei Xia, Shunqing Xu, Yuanyuan Li,