کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491308 | 43410 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced production of n-alkanes in Escherichia coli by spatial organization of biosynthetic pathway enzymes
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کلمات کلیدی
IPTGNADPHACPABDCOAAlkanes - آلکین هاisopropyl β-D-1-thiogalactopyranoside - ایزوپروپیل β-D-1-thiogalactopyranosideSynthetic biology - سنتتیک بیولوژیBiofuel - سوخت های زیستیLuria Bertani - لوریا برتانیnicotinamide adenine dinucleotide phosphate (reduced) - نیکوتین آمید adenine dinucleotide phosphate (کاهش)acyl carrier protein - پروتئین حامل آکیلcoenzyme A - کوآنزیم A
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Alkanes chemically mimic hydrocarbons found in petroleum, and their demand as biofuels is steadily increasing. Biologically, n-alkanes are produced from fatty acyl-ACPs by acyl-ACP reductases (AARs) and aldehyde deformylating oxygenases (ADOs). One of the major impediments in n-alkane biosynthesis is the low catalytic turnover rates of ADOs. Here, we studied n-alkane biosynthesis in Escherichia coli using a chimeric ADO-AAR fusion protein or zinc finger protein-guided ADO/AAR assembly on DNA scaffolds to control their stoichiometric ratios and spatial arrangements. Bacterial production of n-alkanes with the ADO-AAR fusion protein was increased 4.8-fold (24Â mg/L) over a control strain expressing ADO and AAR separately. Optimal n-alkane biosynthesis was achieved when the ADO:AAR binding site ratio on a DNA scaffold was 3:1, yielding an 8.8-fold increase (44Â mg/L) over the control strain. Our findings indicate that the spatial organization of alkane-producing enzymes is critical for efficient n-alkane biosynthesis in E. coli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 192, Part A, 20 December 2014, Pages 187-191
Journal: Journal of Biotechnology - Volume 192, Part A, 20 December 2014, Pages 187-191
نویسندگان
Ziaur Rahman, Bong Hyun Sung, Ji-Yeun Yi, Le Minh Bui, Jun Hyoung Lee, Sun Chang Kim,