کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6491414 43411 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A redesigned Escherichia coli triosephosphate isomerase restores growth properties in a bacterial strain useful for Immobilized Metal Affinity Chromatography (IMAC)
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A redesigned Escherichia coli triosephosphate isomerase restores growth properties in a bacterial strain useful for Immobilized Metal Affinity Chromatography (IMAC)
چکیده انگلیسی
The bacterium Escherichia coli is one of the most commonly used organisms in biotechnology for recombinant protein production and high-throughput development of biopharmaceuticals. The focus of this article is the utilization of proteome based data to design an E. coli expression strain that is improved for initial protein capture via Immobilized Metal Affinity Chromatography (IMAC). Proteome data was specifically applied to guide the modification of a known IMAC binding protein, triosephosphate isomerase (tpiA gene product), and the use of site directed mutagenesis eliminated binding properties. The designer tpiA gene, when reintroduced into an E. coli strain deficient in this enzyme activity, produced a functional protein lacking in surface exposed histidine and was able to restore glycolytic function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 188, 20 October 2014, Pages 48-52
نویسندگان
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