کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6491468 43417 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of oxygen transfer in a 24-well microbioreactor system and potential respirometric applications
ترجمه فارسی عنوان
تشخیص انتقال اکسیژن در یک سیستم میکروبیوراکتور 24 نفره و کاربرد بالقوه تنفسی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The assessment of microbial processes is often done in Microbioreactor systems (MBRs), which allow for parallel cultivation in multiple independent wells. MBRs often include dissolved oxygen sensors, which are convenient for process characterization through oxygen uptake rate and other respirometric determinations. In order to assess respirometric potential of MBRs, a complete assessment of the DO fluorescent quenching sensors was done, showing that they presented a typical error of 0.56%, a signal to noise ratio of 189, a response time from 5.7 to 7.2 s and no drift over a period of 24 h. Then, KLa in the MBR was measured with different cassette and cap designs, liquid volumes, agitation rates, gas flow rates, temperatures and ionic strengths. KLa ranged from 8 to 90 h−1, with a standard deviation between replicates from 2.8 to 17.5%. From these results and a numerical simulation, it was shown that the MBR tested allow the determination of oxygen uptake rates in a range from 0.038 to 3390 mg L−1 h−1, with a determination error less than 15%. Besides OUR determination, it was concluded that the MBR tested is also a convenient tool for dynamic pulse respirometry methods, based on experimental confirmation with four different cultures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 186, 30 September 2014, Pages 58-65
نویسندگان
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