Heterologous pathway for the production of l-phenylglycine from glucose by E. coli

- l-Phenylglycine (l-Phg) was firstly produced from glucose by fermentation.
- Three enzymes from actinomyces were expressed in E. coli and purified.
- New cycle multi-enzyme cascade reactions were developed for l-Phg production.
- Optimizing gene expression and transamination increased l-Phg yield by 224-fold.