کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491638 | 43425 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of acetoin reductases involved in 2,3-butanediol pathway in Klebsiella oxytoca
ترجمه فارسی عنوان
شناسایی ردوکتای آکتئین در مسیر 2،3-بوتانیدیول در کلبسیلا اکسیتوکا
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The acetoin reductase (AR) of Klebsiella oxytoca is responsible for converting acetoin into 2,3-butanediol (2,3-BDO) during sugar fermentation. Deleting the AR encoding gene (budC) in the 2,3-BDO operon does not block production of 2,3-BDO, as another similar gene exists in addition to budC called diacetyl/acetoin reductase (dar) which shares 53% identity with budC. In the present study, both budC and dar of K. oxytoca were independently cloned and expressed in Escherichia coli along with budA (acetolactate decarboxylase) and budB (acetolactate synthase), which are responsible for converting pyruvate into acetoin. The recombinant E. coli expressing budABC and budAB-dar produced 2,3-BDO from glucose but E. coli expressing only budAB did not and produced acetoin alone. This demonstrates that Dar functions similar to BudC. Mutants of budC, dar, and both genes together were developed in K. oxytoca ÎldhA (lactate dehydrogenase). K. oxytoca ÎldhA ÎbudC Îdar, deficient in both AR genes, showed reduced 2,3-BDO concentration when compared to K. oxytoca ÎldhA and K. oxytoca ÎldhA ÎbudC by 84% and 69%, respectively. Interestingly, K. oxytoca ÎldhA Îdar resulted in a significant reduction in the reversible conversion of 2,3-BDO into acetoin than that of K. oxytoca ÎldhA, which was observed in a glucose depleted fermentation culture. In addition, we observed that Dar played a key role in dissimilation of 2,3-BDO in media containing 2,3-BDO alone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 172, 20 February 2014, Pages 59-66
Journal: Journal of Biotechnology - Volume 172, 20 February 2014, Pages 59-66
نویسندگان
Taek Ho Yang, Chelladurai Rathnasingh, Hee Jong Lee, Doyoung Seung,