کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491830 | 43448 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis
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کلمات کلیدی
BBHGammaproteobacteriaAlpha-cyano-4-hydroxycinnamic acidOTU - OTCHCA - آنها می خواهندdalton - دالتونMatrix-assisted laser desorption ionization time-of-flight mass spectrometry - طیف سنجی جرمی یونیزاسیون یونیزاسیون لیزر جذب ماتریسMALDI-TOF MS - مالدی توف MSoperational taxonomic unit - واحد تاکسونومی عملیاتیWheat - گندم
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis](/preview/png/6491830.png)
چکیده انگلیسی
Whole-cell mass spectrometry analysis is a powerful tool to rapidly identify microorganisms. Several studies reported the successful application of this technique to identify a variety of bacterial species with a discriminatory power at the strain level, mainly for bacteria of clinical importance. In this study we used matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to assess the diversity of wheat-associated bacterial isolates. Wheat plants cultivated in non-sterile vermiculite, under greenhouse conditions were used for bacterial isolation. Total cellular extracts of 138 isolates were analyzed by MALDI-TOF MS and the mass spectra were used to cluster the isolates. Taxonomic identification and phylogenetic reconstruction based on 16S rRNA gene sequences showed the presence of Pseudomonas, Pantoea, Acinetobacter, Enterobacter and Curtobacterium. The 16S rRNA gene sequence analyses were congruent with the clusterization from mass spectra profile. Moreover, MALDI-TOF whole cell mass profiling allowed a finer discrimination of the isolates, suggesting that this technique has the potential of differentiating bacterial isolates at the strain level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 165, Issues 3â4, 10 June 2013, Pages 167-174
Journal: Journal of Biotechnology - Volume 165, Issues 3â4, 10 June 2013, Pages 167-174
نویسندگان
Maria Isabel Stets, Artur Soares Jr., Luciano Fernandes Huergo, Emanuel Maltempi de Souza, Vandeir Francisco Guimarães, Alexessander Couto Alves, Maria Berenice Reynaud Steffens, Rose Adele Monteiro, Fábio de Oliveira Pedrosa, Leonardo Magalhães Cruz,