Short-term dynamic behavior of Escherichia coli in response to successive glucose pulses on glucose-limited chemostat cultures

► Transient E. coli behaviors in response to repeated glucose pulses of different intensities and frequencies were investigated. ► Macroscopic and transcriptional transient E. coli responses were monitored. ► Short-term specific kinetics data were determined and discussed. ► Transient metabolic shifts were monitored in real-time using E. coli yciG::luxCDABE.