Bioconversion of major ginsenosides Rg1 to minor ginsenoside F1 using novel recombinant ginsenoside hydrolyzing glycosidase cloned from Sanguibacter keddieii and enzyme characterization

► A recombinant ginsenoside hydrolyzing glycosidase (BglSk) was constructed for the biotransformation of major ginsenosides. ► BglSk has very diverse biotransformation ability against ginsenosides. ► BglSk could produce ginsenoside F1 from ginsenoside Rg1.