Mathematical approach for the optimal expansion of erythroid progenitors in monolayer culture

► We examine culture conditions for inoculation and passaging of erythroid progenitors. ► We use mathematical expressions to define the growth curves of peripheral blood derived erythroid progenitors over the course of their expansion process. ► Significantly different growth curves are obtained at each passage confirming the altered growth kinetics of erythroid progenitors during sequential passaging in expansion process. ► Cost analysis suggest a 60 h passage length at every passage, resulting in a 43% process-cost reduction.