A novel strategy to over-express and purify homologous proteins from Streptococcus pneumoniae

► A new method to over-express and purify homologous proteins from Streptococcus pneumoniae. ► Combined use of the plasmid pMU1328 and the pneumococcal promoter P96. ► Expression of secreted, membrane or cell wall proteins, wild type or mutant. ► Concurrent assessment of protein functionality and purification. ► Homologous pneumococcal RrgBHis purification and characterization.