Chemiluminescent DNA optical fibre sensor for Brettanomyces bruxellensis detection

► Optimisation of a chemiluminescent optical fibre biosensor for microbial detection. ► Two types of DNA probes, capture probe and secondary probe, were designed. ► Capture probe specificity and sensitivity were determined using the dot blot technique. ► Fibre hybridisation steps were optimised to detect the presence of B. bruxellensis. ► Results demonstrated a rapid sensitive and semi-quantitative optical fibre immunoassay.