Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae

► Hyper mannosylation inhibits cellulosome assembly in Saccharomyces cerevisiae. ► We examined cellulosome assembly using dockerin-type cellulases secreted glycosylation mutants. ► Disruption of glycosylation genes located in the endoplasmic reticulum enhanced cellulosome assembly intensely.