Coupling of permeabilized cells of Gluconobacter oxydans and Ralstonia eutropha for asymmetric ketone reduction using H2 as reductant

► Coupled use of two permeabilized cell catalysts for stereoselective ketone reduction. ► Higher reaction rate and higher yield in the two-cell system compared to a substrate coupled approach. ► NADH regeneration using hydrogenase/hydrogen does not give any side products.