کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6492258 | 43523 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery](/preview/png/6492258.png)
چکیده انگلیسی
Baculoviruses can express transgenes under mammalian promoters in a wide range of vertebrate cells. However, the success of transgene expression is dependent on both the appropriate cell type and culture conditions. We studied the mechanism behind the substantial effect of the cell culture medium on efficiency of the baculovirus transduction in different cell lines. We tested six cell culture mediums; the highest transduction efficiency was detected in the presence of RPMI 1640 medium. Vimentin, a major component of type III intermediate filaments, was reorganized in the optimized medium, which associated with enhanced nuclear entry of baculoviruses. Accordingly, the phosphorylation pattern of vimentin was changed in the studied cell lines. These results suggest that vimentin has an important role in baculovirus entry into vertebrate cells. Enhanced gene delivery in the optimized medium was observed also with adenoviruses and lentiviruses. The results highlight the general importance of the culture medium in the assembly of the cytoskeleton network and in viral gene delivery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 145, Issue 2, 15 January 2010, Pages 111-119
Journal: Journal of Biotechnology - Volume 145, Issue 2, 15 January 2010, Pages 111-119
نویسندگان
Anssi J. Mähönen, Kaisa-Emilia Makkonen, Johanna P. Laakkonen, Teemu O. Ihalainen, Sari P. Kukkonen, Minna U. Kaikkonen, Maija Vihinen-Ranta, Seppo Ylä-Herttuala, Kari J. Airenne,