کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6492466 | 1417578 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel fluorescence “turn off-on” nano-sensor for detecting Cu2+ and Cysteine in living cells
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, a novel fluorescent sensor (quercetin-Pluronic-F127 nano-micelles) was prepared by the facile self-assembly method with natural quercetin (Qc) and Pluronic-F127 (F127). Qc-F127 nano-micelles were characterized by TEM which indicated that Qc have successfully entered into the amphiphilic micelle. The nano micelles with sizes of 25 to 40â¯nm showed a strong green fluorescence (FL). The sensor exhibits high sensitivity and selectivity towards Cu2+ with a linear range of 5â¯Ãâ¯10â8-4â¯Ãâ¯10â6â¯molâ¯Lâ1, the detection limit of 1â¯Ãâ¯10â8â¯molâ¯L â1. Moreover, the Qc-F127 nano-micelles can be used to detect Cu2+ in vitro due to their good biocompatibility. Besides Cu2+ sensing, when Cysteine (Cys) were added, the FL intensity of Qc-F127-Cu(II) system could be restored due to the chelation interactions between Cu2+ ions and the sulfydryl and amino groups of Cys. So, Qc-F127-Cu(II) system can used to detect Cys, and the linear range and the limit of detection of the Qc-F127-Cu2+ system were 3â¯Ãâ¯10â7-3.5â¯Ãâ¯10â5â¯molâ¯Lâ1 and 6â¯Ãâ¯10â8â¯molâ¯L â1. Moreover, Qc-F127-Cu(II) has been used for imaging of Cys in cells, which demonstrates its values for potential application in biological systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 362, 1 July 2018, Pages 14-20
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 362, 1 July 2018, Pages 14-20
نویسندگان
Weina Jiang, Shilong Yang, Wen Lu, Buhong Gao, Li Xu, Xu Sun, Dong Jiang, Hai-Jun Xu, Mengtao Ma, Fuliang Cao,