کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494209 | 44798 | 2016 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii
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کلمات کلیدی
CDWT2PSesquiterpenoidsGGPPYFPChlamydomonas reinhardtiiPCPsCFPGPPFPPubiquinoneTerpenoids - ترپنوئیدTris acetate phosphate medium - تری استات فسفات متوسطMicroalgae - ریز جلبک هاTAP - ضربه زدنfarnesyl diphosphate - فارسیل دی فسفاتcell dry weight - وزن خشک سلولیPatchoulol - پچولولGeranyl diphosphate - ژانایل دی فسفاتgeranylgeranyl diphosphate - گوارانیلگرانیل دی فسفات
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii](/preview/png/6494209.png)
چکیده انگلیسی
The heterologous expression of terpene synthases in microbial hosts has opened numerous possibilities for bioproduction of desirable metabolites. Photosynthetic microbial hosts present a sustainable alternative to traditional fermentative systems, using freely available (sun)light and carbon dioxide as inputs for bio-production. Here, we report the expression of a patchoulol synthase from Pogostemon cablin Benth in the model green microalga Chlamydomonas reinhardtii. The sesquiterpenoid patchoulol was produced from the alga and was used as a marker of sesquiterpenoid production capacity. A novel strategy for gene loading was employed and patchoulol was produced up to 922±242 µg gâ1 CDW in six days. We additionally investigated the effect of carbon source on sesquiterpenoid productivity from C. reinhardtii in scale-up batch cultivations. It was determined that up to 1.03 mg Lâ1 sesquiterpenoid products could be produced in completely photoautotrophic conditions and that the alga exhibited altered sesquiterpenoid production metabolism related to carbon source.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 38, November 2016, Pages 331-343
Journal: Metabolic Engineering - Volume 38, November 2016, Pages 331-343
نویسندگان
Kyle J. Lauersen, Thomas Baier, Julian Wichmann, Robin Wördenweber, Jan H. Mussgnug, Wolfgang Hübner, Thomas Huser, Olaf Kruse,