کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494319 | 44805 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum
ترجمه فارسی عنوان
تکامل آزمایشگاهی انطباق یافته بوسیسورسور تولید لولین در کورینباکتریوم گلوتامیکوم
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Adaptive laboratory evolution has proven a valuable strategy for metabolic engineering. Here, we established an experimental evolution approach for improving microbial metabolite production by imposing an artificial selective pressure on the fluorescent output of a biosensor using fluorescence-activated cell sorting. Cells showing the highest fluorescent output were iteratively isolated and (re-)cultivated. The l-valine producer Corynebacterium glutamicum ÎaceE was equipped with an L-valine-responsive sensor based on the transcriptional regulator Lrp of C. glutamicum. Evolved strains featured a significantly higher growth rate, increased l-valine titers (~25%) and a 3-4-fold reduction of by-product formation. Genome sequencing resulted in the identification of a loss-of-function mutation (UreD-E188*) in the gene ureD (urease accessory protein), which was shown to increase l-valine production by up to 100%. Furthermore, decreased l-alanine formation was attributed to a mutation in the global regulator GlxR. These results emphasize biosensor-driven evolution as a straightforward approach to improve growth and productivity of microbial production strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 32, November 2015, Pages 184-194
Journal: Metabolic Engineering - Volume 32, November 2015, Pages 184-194
نویسندگان
Regina Mahr, Cornelia Gätgens, Jochem Gätgens, Tino Polen, Jörn Kalinowski, Julia Frunzke,