کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494320 | 44805 | 2015 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment of bacterial acyltransferases for an efficient lipid production in metabolically engineered strains of E. coli
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کلمات کلیدی
FFATAGfatty acid methyl ester(s)TFATLCCDWFAEEfatty acid ethyl ester - اتیل استر اسید چربfree fatty acid(s) - اسید چرب آزاد (ها)Escherichia coli - اشریشیا کُلیtriacylglycerol - تری آسیل گلیسرول diacylglycerol - دیسیل گلیسیرینDAG - روزBiofuel - سوخت های زیستیFAME - فامcell dry weight - وزن خشک سلولیthin-layer chromatography - کروماتوگرافی نازک لایه
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Assessment of bacterial acyltransferases for an efficient lipid production in metabolically engineered strains of E. coli Assessment of bacterial acyltransferases for an efficient lipid production in metabolically engineered strains of E. coli](/preview/png/6494320.png)
چکیده انگلیسی
Microbially produced lipids like triacylglycerols or fatty acid ethyl esters are currently of great interest as fuel replacements or other industrially relevant compounds. They can even be produced by non-oleaginous microbes, like Escherichia coli, upon metabolic engineering. However, there is still much room for improvement regarding the yield for a competitive microbial production of lipids or biofuels. We genetically engineered E. coli by expressing fadD, fadR, pgpB, plsB and 'tesA in combination with atfA from Acinetobacter baylyi. A total fatty acid contents of up to 16% (w/w) was obtained on complex media, corresponding to approximately 9% (w/w) triacylglycerols and representing the highest titers of fatty acids and triacylglycerols obtained in E. coli under comparable cultivation conditions, so far. To evaluate further possibilities for an optimization of lipid production, ten promising bacterial wax ester synthase/acyl-Coenzyme A:diacylglycerol acyltransferases were tested and compared. While highest triacylglycerol storage was achieved with AtfA, the mutated variant AtfA-G355I turned out to be most suitable for fatty acid ethyl ester biosynthesis and enabled an accumulation of approx. 500Â mg/L without external ethanol supplementation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 32, November 2015, Pages 195-206
Journal: Metabolic Engineering - Volume 32, November 2015, Pages 195-206
نویسندگان
Annika Röttig, Paul Jannis Zurek, Alexander Steinbüchel,