کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494606 | 44812 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Conversion of lignocellulosic material to ethanol is a major challenge in second generation bio-fuel production by yeast Saccharomyces cerevisiae. This report describes a novel strategy named “two-stage transcriptional reprogramming (TSTR)” in which key gene expression at both glucose and xylose fermentation phases is optimized in engineered S. cerevisiae. Through a combined genome-wide screening of stage-specific promoters and the balancing of the metabolic flux, ethanol yields and productivity from mixed sugars were significantly improved. In a medium containing 50Â g/L glucose and 50Â g/L xylose, the top-performing strain WXY12 rapidly consumed glucose within 12Â h and within 84Â h it consistently achieved an ethanol yield of 0.48Â g/g total sugar, which was 94% of the theoretical yield. WXY12 utilizes a KGD1 inducible promoter to drive xylose metabolism, resulting in much higher ethanol yield than a reference strain using a strong constitutive PGK1 promoter. These promising results validate the TSTR strategy by synthetically regulating the xylose assimilation pathway towards efficient xylose fermentation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 24, July 2014, Pages 150-159
Journal: Metabolic Engineering - Volume 24, July 2014, Pages 150-159
نویسندگان
Limin Cao, Xingliang Tang, Xinyuan Zhang, Jingtao Zhang, Xuelei Tian, Jingyu Wang, Mingyong Xiong, Wei Xiao,