CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion

► “Difficult-to-express” proteins consistently yield low expression from mammalian cells. ► Low protein secretion can stem from faulty cleavage and precipitation in degradation organelles. ► Expression of translational arrest and secretion pathway components restores proper secretion. ► Secretion pathway engineering also increases expression of an easy-to-express immunoglobulin. ► Metabolic engineering thus resolves CHO cell protein processing and secretion bottlenecks.