Characterization of a thermostable inulin fructotransferase from Clostridium clostridioforme AGR2157 that produces difructose dianhydride I from inulin

- DFA I-producing IFTase was characterized from C. clostridioforme.
- The enzyme showed the maximum activity at 50 °C and pH 5.5.
- The enzyme was stable under heat treatment of up to 80 °C for 4 h.
- The minor products were GF, GF2, GF3, and GF4.
- The smallest substrate was GF3.