High throughput screening of monoamine oxidase (MAO-N-D5) substrate selectivity and rapid kinetic model generation

- Simultaneous microscale substrate screening and kinetic characterization.
- Selective oxidation of secondary amines in 8 and 9 carbon rings.
- Formation of new heterocyclic rings for selected substrates oxidized by MAO-N-D5.
- Development of the first kinetic model for the MAO-N-D5.
- Model predictions for positive substrates were verified experimentally.
- Optimum conditions for bioprocess design were identified.