Characterization of a novel hormone-sensitive lipase family esterase from Rhizomucor miehei with tertiary alcohol hydrolysis activity

- A novel esterase gene from Rhizomucor miehei was cloned and expressed in E. coli.
- The recombinant esterase was purified and biochemically characterized.
- The enzyme was highly stable in high concentrations of organic solvents.
- The esterase efficiently hydrolyzed sterically hindered esters of tertiary alcohols.
- The study presents a novel fungal HSL family esterase with potential applications.