Characterization of an enantioselective amidase from Cupriavidus sp. KNK-J915 (FERM BP-10739) useful for enzymatic resolution of racemic 3-piperidinecarboxamide

- An S-specific N-benzyl-3-piperidinecarboxamide-hydrolyzing enzyme was purified from Cupriavidus sp. KNK-J915.
- The amidase was highly S-selective for both N-benzyl-3-piperidinecarboxamide and 3-piperidinecarboxamide.
- The gene for the enantioselective amidase was cloned.
- The amidase was expressed in Escherichia coli.