Purification and covalent immobilization of benzaldehyde lyase with heterofunctional chelate-epoxy modified magnetic nanoparticles and its carboligation reactivity

- Magnetic nanoparticles with epoxy-chelate ligands were prepared for one-step purification and covalent immobilization of crude histidine-tagged recombinant benzaldehyde lyase from Pseudomonas fluorescens Biovar I.
- We used them as a heterogenous biocatalyst for benzoin condensation reaction.
- The product which is (R)-benzoin was obtained in high chemical and optical yields.
- Additional advantages are its reusability and it is easy to work with.