Differential properties of native and tagged or untagged recombinant glucose isomerases of Streptomyces sp. SK and possible implication of the glycosylation

- Biochemical properties of Streptomyces sp. SK glucose isomerase SKGI and its two recombinant forms were compared.
- Native SKGI was the most thermostable compared to the two recombinant enzymes.
- His tagged SKGI is less thermostable/thermoactive than untagged recombinant SKGI.
- Possible O-glycosylation of native SKGI (8%) at position Thr6 and/or Thr30.
- The His tag is located at the dimerization interface promoting the dimer dissociation.