Characterization of chemo- and regioselectivity in enzyme-catalyzed consecutive hydrolytic deprotection of methyl acetyl derivatives of 1-β-O-acyl glucuronides

▶ Enzyme-catalyzed one-pot procedure for synthesis of 1-β-O-acyl glucuronides. ▶ LAS and CSR play complementary and synergistic roles in chemoselective O-deacetylation. ▶ LAS shows high regioselective 3-O-deacetylation activity. ▶ Differences in acyl preferences between LAS and CSR toward p-nitrophenyl esters.