کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6553237 | 1422143 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dual amplification strategy for improved efficiency of forensic DNA analysis using NGM Detectâ¢, NGM⢠or Globalfiler⢠kits
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Dual amplification strategy for improved efficiency of forensic DNA analysis using NGM Detectâ¢, NGM⢠or Globalfiler⢠kits Dual amplification strategy for improved efficiency of forensic DNA analysis using NGM Detectâ¢, NGM⢠or Globalfiler⢠kits](/preview/png/6553237.png)
چکیده انگلیسی
The new generation of STR amplification kits with improved sensitivity and additional genetic markers is designed particularly for analyzing difficult traces with a high DNA degradation index, presence of inhibitors and low level of DNA. In the new NGM Detect⢠kit, modifications including changing the primers' sequences and shortening of STR markers are introduced. The quality control system (IQCS, IQCL) used to detect DNA degradation and the presence of inhibitors in the sample is an additional feature. The purpose of this study was to compare the results of analysis of different kinds of typical casework samples obtained using NGM⢠or GlobalFiler⢠kits with those generated using the new NGM Detect⢠kit. The results indicate that the tested kit is particularly useful for the analysis of challenging samples for which incomplete profiles are generated with the NGM⢠or GlobalFiler⢠kits. The increased number of positively typed alleles gives better statistical parameters in genetic identification cases. We conclude that the NGM Detect⢠kit can be recommended for the double amplification protocol together with the NGM or GlobalFiler⢠kits.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 35, July 2018, Pages 46-49
Journal: Forensic Science International: Genetics - Volume 35, July 2018, Pages 46-49
نویسندگان
Agnieszka Parys-Proszek, Maria Wróbel, Magdalena MarciÅska, Tomasz Kupiec,