کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6553900 | 160890 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
FACS separation of non-compromised forensically relevant biological mixtures
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Although focusing attention on the statistical analysis of complex mixture profiles is important, the forensic science community will also benefit from directing research to improving the reduction of the incidence of mixtures before DNA extraction. This preliminary study analysed the use of fluorescence assisted cell sorting (FACS) for separation of cellular mixtures before DNA extraction, specifically mixtures of relatively fresh blood and saliva from two donors, prepared in 14 different mixture ratios. Improvements in the number of detectable alleles from the targeted cell type and overall profile quality were seen when compared to the results from unseparated samples. STRmix calculations revealed increases in likelihood ratios after separation, demonstrating the potential for higher probative values to be obtained from forensically relevant mixtures after subjecting them to FACS than from unsorted samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 14, January 2015, Pages 194-200
Journal: Forensic Science International: Genetics - Volume 14, January 2015, Pages 194-200
نویسندگان
Timothy J. Verdon, R. John Mitchell, Weisan Chen, Kun Xiao, Roland A.H. van Oorschot,