کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6554161 160901 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of internal control sequence and length on the response to PCR inhibition in real-time PCR quantitation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
The effect of internal control sequence and length on the response to PCR inhibition in real-time PCR quantitation
چکیده انگلیسی
PCR inhibitors can originate from a variety of sources and can co-extract with the DNA template, resulting in reduced amplification and/or dropped alleles. Currently real time PCR is used to provide a check for the presence of PCR inhibition by monitoring the quality of amplification of an internal control. In this paper we examine the effect of internal control length and sequences on its sensitivity to PCR inhibition by varying concentrations of commonly encountered PCR inhibitors. Data from both amplification and melt curves were evaluated. The results show that while amplicon sequence has minor effects on amplification efficiency and melt curves, amplicon length has a more dramatic effect, regardless of inhibitor type. Given the increasing variety of STR typing kits and their documented differences in performance with respect to inhibition, the data obtained in this study can be used to assist designers of real time PCR kits to adjust their internal PCR controls (IPC) to permit a more targeted estimation of inhibition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 9, March 2014, Pages 55-60
نویسندگان
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