Involvement of ligninolytic enzymes of Myceliophthora vellerea HQ871747 in decolorization and complete mineralization of Reactive Blue 220

Myceliophthora vellerea HQ871747 was isolated from decayed wood sample and was subjected to phylogenetic analysis by using basidiomycete-specific ITS primers. Preferable physicochemical parameters were identified to achieve maximum enzyme and decolorization efficiency. M. vellerea HQ871747 tolerated 20 g L−1 NaCl concentration without decrease in average rate of decolorization and showed more than 50% decolorization up to 7th cycle of repetitive dye addition. It is also able to degrade mixture of various reactive, acidic, direct and disperse dyes. In immobilized packed-bed reactor 29-65 mg L−1 h−1 decolorization rate was achieved with >80% COD removal, 154 and 75 UL−1 MnP and laccase activity respectively. Purified MnP and laccase were monomeric protein with corresponding 37 and 25 kDa size on the SDS-PAGE. In this study it is proposed that the asymmetric cleavage of RB220 and HPLC, NMR and GC-MS confirmed formation of 2-diazo benzene sulfonic acid, naphthalene-2-sulfonic acid and 4-(4-diazenyl-5-oxo-4, 5-dihydro-1H-pyrazol-1-yl) benzene sulfonic acid. Treated wastewater was found to stimulate the growth of selected soil bacteria as well as plant growth.