Rational engineering of synergically stabilized aptamer-cDNA duplex probes for strand displacement based electrochemical sensors

A class of synergically stabilized aptamer-cDNA duplex probe (B) is engineered by integrating a short aptamer-involved (AI) base pair sequence and a short additional (SA) DNA sequence. The synergically stabilized probe possesses the advantage of less aptamer bases paired to the cDNA bases over the conventional probe (A, the duplex is made up entirely of AI base pairs), which provides significantly enhanced response rates of electrochemical aptamer sensors based on target-induced strand displacement molecular recognitions.124