کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6602950 | 1424083 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toehold-mediated strand displacement reaction triggered by nicked DNAzymes substrate for amplified electrochemical detection of lead ion
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Searching for a sensitive analytical method for the detection of lead ion (Pb2+) without involving any enzymes is of great significance in environmental monitoring. Herein, based on the integration of toehold-mediated strand displacement reaction (TSDR) and the electrocatalysis of magnetic Fe3O4 toward electron mediator methylene blue (MB) for signal amplification, a sensitive electrochemical biosensor for Pb2+ was developed by using Pb2+-specific DNAzymes as recognition probe. The electrode surface was firstly modified with Au nanoparticles (AuNPs)-loaded Fe3O4 nanocomposites (AuNPs@Fe3O4) and a DNA duplex containing the capture probe (NH2-CP) with two specific toehold sequences. Fuelled by the cleaved substrate fragments (rSS) of Pb2+-specific DNAzymes, TSDR was activated to allow for the complete assembly of MB-labelled signal probe (MB-SP) through the hybridization with NH2-CP, resulting in the spatial proximity of MB close to Fe3O4 in the modified electrode surface. In this case, the amplified electrochemical output signal was further enhanced due to the robust electrocatalysis of Fe3O4 toward MB reduction. Thus, the developed sensitive biosensor for Pb2+ exhibited a reliable linear range of 1 pM-500â¯nM with the limit of detection of 0.32 pM. Importantly, by changing the specific DNAzymes, the proposed assay platform would be easily extended to quantitative detection of other metal ions without involving any enzymes and would be potential and promising for environment monitoring.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 274, 1 June 2018, Pages 16-22
Journal: Electrochimica Acta - Volume 274, 1 June 2018, Pages 16-22
نویسندگان
Jianmin Zhao, Ting Zheng, Jiaxi Gao, Wenju Xu,