کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6609810 | 459548 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impedance sensor for rapid enumeration of E. coli in milk samples
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Detection of pathogenic bacteria in dairy farms and processing plants is important for milk quality control. In this study, a simple in situ method for determining the bacterial concentration in milk was developed with an interdigitated microelectrode sensor. This method was based on a punctual measurement of the electrode-milk interface impedance change due to bacterial metabolism in the milk. The interface impedance varied predominately at low frequencies, attributed to the change on the double-layer capacitance. The detection time TD, defined as the time required to attain a â10% impedance change at 10Â Hz, became a practicable means for the estimation of initial E. coli concentration in milk samples. Regression analysis resulted in a third-order polynomial relationship between the detection time and the logarithm of the initial E. coli concentration (N0, cells/ml) in a milk sample, i.e., log N0Â =Â 11.48Â -Â 2.33 TDÂ +Â 0.288(TD)2Â -Â 0.0154(TD)3 with R2Â =Â 0.9988. The sensor was able to detect E. coli in milk of initial concentrations as low as 7Â cells/ml. The proposed impedance sensor is suitable for determining bacterial contamination at dairy farms and processing plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 182, 10 November 2015, Pages 89-95
Journal: Electrochimica Acta - Volume 182, 10 November 2015, Pages 89-95
نویسندگان
Jen-Tsai Liu, Kalpana Settu, Jang-Zern Tsai, Ching-Jung Chen,