کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6609810 | 459548 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impedance sensor for rapid enumeration of E. coli in milk samples
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Impedance sensor for rapid enumeration of E. coli in milk samples Impedance sensor for rapid enumeration of E. coli in milk samples](/preview/png/6609810.png)
چکیده انگلیسی
Detection of pathogenic bacteria in dairy farms and processing plants is important for milk quality control. In this study, a simple in situ method for determining the bacterial concentration in milk was developed with an interdigitated microelectrode sensor. This method was based on a punctual measurement of the electrode-milk interface impedance change due to bacterial metabolism in the milk. The interface impedance varied predominately at low frequencies, attributed to the change on the double-layer capacitance. The detection time TD, defined as the time required to attain a â10% impedance change at 10Â Hz, became a practicable means for the estimation of initial E. coli concentration in milk samples. Regression analysis resulted in a third-order polynomial relationship between the detection time and the logarithm of the initial E. coli concentration (N0, cells/ml) in a milk sample, i.e., log N0Â =Â 11.48Â -Â 2.33 TDÂ +Â 0.288(TD)2Â -Â 0.0154(TD)3 with R2Â =Â 0.9988. The sensor was able to detect E. coli in milk of initial concentrations as low as 7Â cells/ml. The proposed impedance sensor is suitable for determining bacterial contamination at dairy farms and processing plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 182, 10 November 2015, Pages 89-95
Journal: Electrochimica Acta - Volume 182, 10 November 2015, Pages 89-95
نویسندگان
Jen-Tsai Liu, Kalpana Settu, Jang-Zern Tsai, Ching-Jung Chen,