کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6615351 459622 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of electrochemical impedance spectroscopy for studying of enzyme kinetics
ترجمه فارسی عنوان
استفاده از طیف سنجی امپدانس الکتروشیمیایی برای مطالعه سینتیک آنزیم
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
The application of electrochemical impedance spectroscopy (EIS) in theory and experiment for investigation of redox enzyme kinetics has been described. As a model system an enzyme horseradish peroxidase adsorbed on graphite electrode has been chosen. Three different mathematical models based on generalized mechanism of horseradish peroxidase catalyzed hydrogen peroxide reduction have been formulated and used for derivation of theoretical electrochemical impedances. In this way, mechanistic details related to bioelectrochemical reaction including all relevant kinetic parameters are obtained. The presented approach overcomes limitations of classical equivalent circuit approach, since it does not rely on phenomenological elements, but on particular reaction mechanism. We have shown that the EIS is more sensitive for parameter estimation and model discrimination than a steady state response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 110, 1 November 2013, Pages 94-104
نویسندگان
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